Overexpression of SoCYP85A1 Increases the Accumulation of Castasterone and Confers Enhanced Black Shank Tolerance in Tobacco Through Modulation of the Antioxidant Enzymes' Activities.
Identifieur interne : 000431 ( Main/Exploration ); précédent : 000430; suivant : 000432Overexpression of SoCYP85A1 Increases the Accumulation of Castasterone and Confers Enhanced Black Shank Tolerance in Tobacco Through Modulation of the Antioxidant Enzymes' Activities.
Auteurs : Fangmeng Duan [République populaire de Chine] ; Wenwen Song [République populaire de Chine]Source :
- Frontiers in plant science [ 1664-462X ] ; 2019.
Abstract
Black shank caused by Phytophthora nicotianae is one of the most devastating diseases in tobacco production. In this study, we characterized a novel cytochromic resistance gene, SoCYP85A1, from spinach, which was upregulated in response to P. nicotianae infection. Overexpression of SoCYP85A1 in tobacco resulted in remarkable resistance to pathogen inoculation, with diverse resistance levels in different transgenic lines. Meanwhile, a significant accumulation of castasterone (CS) was detected in transgenic plants when challenged with the pathogen. Moreover, activities of antioxidant enzymes were enhanced by SoCYP85A1 in the transgenic lines as compared to those in the wild types inoculated with P. nicotianae. In addition, the alteration of CS content resulted in interference of phytohormone homeostasis. Overall, these results demonstrate that SoCYP85A1 can participate in the defense response to P. nicotianae through the involvement of defense enzymes and by interaction with certain phytohormones. Our findings suggest that SoCYP85A1 could be used as a potential candidate gene for improving resistance to black shank disease in tobacco and other economic crops.
DOI: 10.3389/fpls.2019.00349
PubMed: 30984218
PubMed Central: PMC6448038
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Overexpression of <i>SoCYP85A1</i> Increases the Accumulation of Castasterone and Confers Enhanced Black Shank Tolerance in Tobacco Through Modulation of the Antioxidant Enzymes' Activities.</title><author><name sortKey="Duan, Fangmeng" sort="Duan, Fangmeng" uniqKey="Duan F" first="Fangmeng" last="Duan">Fangmeng Duan</name><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation></author><author><name sortKey="Song, Wenwen" sort="Song, Wenwen" uniqKey="Song W" first="Wenwen" last="Song">Wenwen Song</name><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30984218</idno><idno type="pmid">30984218</idno><idno type="doi">10.3389/fpls.2019.00349</idno><idno type="pmc">PMC6448038</idno><idno type="wicri:Area/Main/Corpus">000501</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000501</idno><idno type="wicri:Area/Main/Curation">000501</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000501</idno><idno type="wicri:Area/Main/Exploration">000501</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Overexpression of <i>SoCYP85A1</i> Increases the Accumulation of Castasterone and Confers Enhanced Black Shank Tolerance in Tobacco Through Modulation of the Antioxidant Enzymes' Activities.</title><author><name sortKey="Duan, Fangmeng" sort="Duan, Fangmeng" uniqKey="Duan F" first="Fangmeng" last="Duan">Fangmeng Duan</name><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation></author><author><name sortKey="Song, Wenwen" sort="Song, Wenwen" uniqKey="Song W" first="Wenwen" last="Song">Wenwen Song</name><affiliation wicri:level="1"><nlm:affiliation>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao</wicri:regionArea><wicri:noRegion>Qingdao</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Black shank caused by <i>Phytophthora nicotianae</i> is one of the most devastating diseases in tobacco production. In this study, we characterized a novel cytochromic resistance gene, <i>SoCYP85A1</i>, from spinach, which was upregulated in response to <i>P. nicotianae</i> infection. Overexpression of <i>SoCYP85A1</i> in tobacco resulted in remarkable resistance to pathogen inoculation, with diverse resistance levels in different transgenic lines. Meanwhile, a significant accumulation of castasterone (CS) was detected in transgenic plants when challenged with the pathogen. Moreover, activities of antioxidant enzymes were enhanced by <i>SoCYP85A1</i> in the transgenic lines as compared to those in the wild types inoculated with <i>P. nicotianae</i>. In addition, the alteration of CS content resulted in interference of phytohormone homeostasis. Overall, these results demonstrate that <i>SoCYP85A1</i> can participate in the defense response to <i>P. nicotianae</i> through the involvement of defense enzymes and by interaction with certain phytohormones. Our findings suggest that <i>SoCYP85A1</i> could be used as a potential candidate gene for improving resistance to black shank disease in tobacco and other economic crops.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30984218</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Overexpression of <i>SoCYP85A1</i> Increases the Accumulation of Castasterone and Confers Enhanced Black Shank Tolerance in Tobacco Through Modulation of the Antioxidant Enzymes' Activities.</ArticleTitle><Pagination><MedlinePgn>349</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2019.00349</ELocationID><Abstract><AbstractText>Black shank caused by <i>Phytophthora nicotianae</i> is one of the most devastating diseases in tobacco production. In this study, we characterized a novel cytochromic resistance gene, <i>SoCYP85A1</i>, from spinach, which was upregulated in response to <i>P. nicotianae</i> infection. Overexpression of <i>SoCYP85A1</i> in tobacco resulted in remarkable resistance to pathogen inoculation, with diverse resistance levels in different transgenic lines. Meanwhile, a significant accumulation of castasterone (CS) was detected in transgenic plants when challenged with the pathogen. Moreover, activities of antioxidant enzymes were enhanced by <i>SoCYP85A1</i> in the transgenic lines as compared to those in the wild types inoculated with <i>P. nicotianae</i>. In addition, the alteration of CS content resulted in interference of phytohormone homeostasis. Overall, these results demonstrate that <i>SoCYP85A1</i> can participate in the defense response to <i>P. nicotianae</i> through the involvement of defense enzymes and by interaction with certain phytohormones. Our findings suggest that <i>SoCYP85A1</i> could be used as a potential candidate gene for improving resistance to black shank disease in tobacco and other economic crops.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Duan</LastName><ForeName>Fangmeng</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Wenwen</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal 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